Links to other online isoelectric point estimation tools, databases, etc.

Databases

• Proteome-pI 2.0 - Proteome Isoelectric Point Database 2.0 (predicted isoelectric point for ~61 million proteins accross 20,115 organisms)
• Proteome-pI - Proteome Isoelectric Point Database (predicted isoelectric point for ~21 million proteins accross 5,029 organisms)
• SWISS-2DPAGE - two-dimensional polyacrylamide gel electrophoresis database - ~2,000 experimental isoelectric points

Isoelectric point calculation methods

• IPC 1.0 Isoelectric Protein Calculator (the first version of IPC based on pKa optimization)
• PROTEIN CALCULATOR v3.3 9
• Compute pI/Mw tool (Bjellqvist)
• ProMoST - Protein Modification Screening Tool

pKa calculation methods

H++ program for protein pKa calculations using the Poisson-Boltzmann approach (structure based)

MCCE Multi-Conformation Continuum Electrostatics (pKa calculation based on structure)

Rosetta pKa (calculation of protein pKa values using Rosetta)

Wikipedia articles

• Isoelectric point (pI)
• pK acid dissociation constant
• Henderson-Hasselbach equation
• Protein pKa calculations

Acknowledgements (aka libraries, tools used):

http server script programming web server front-end CanvasJS JavaScript charts for web server front-end HTML, CSS, and JS templates

deep learning (front-end) deep learning (back-end) statistics (CV, sampling, feature selection, etc.) plotting some scripts

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